This article will explain in detail how to test the content of Anionic polyacrylamide (APAM) from three aspects.The first is to use infrared spectroscopy for testing, then the principle and application of fluorescence colorimetric method are introduced, and the precautions for use in the experiment are mentioned.The principle and operation steps of thin layer chromatography are introduced.Through these test methods, the content of APAM can be accurately determined.

1. Infrared spectroscopy

Infrared spectroscopy is a commonly used method for testing APAM content.This method determines the content of APAM by detecting the characteristic absorption peaks of APAM.During the test, the APAM sample first needs to be prepared into a film, and then scanned using an infrared spectrometer.The content of APAM can be calculated based on the intensity and position of the absorption peak of the sample.

It is worth noting that when performing infrared spectroscopy testing, it is necessary to pay attention to the preparation process of the sample and the operating standards of the instrument used.Attention should also be paid to eliminating interfering factors to ensure the accuracy of the test results.

2. Fluorescence colorimetric method

Fluorescence colorimetry is another commonly used method for testing APAM content.This method uses the fluorescence signal generated after the chemical reaction between APAM and a specific reagent for quantitative analysis.During the test, the reagent and the APAM sample are first mixed, the fluorescence intensity is then measured, and compared with the standard curve to determine the content of APAM.

When using fluorescence colorimetry for testing, attention needs to be paid to selecting suitable reagents and controlling experimental conditions.In addition, pay attention to some details in the preparation and operation of the sample, such as the control of temperature, pH value, etc.

3. Thin layer chromatography

Thin layer chromatography can also be used to test the content of APAM.This method separates and detects APAM samples from other substances on a thin-layer chromatographic plate.According to the comparison of the migration distance of APAM on the thin layer chromatographic plate and the standard curve, the content of APAM can be determined.

When performing thin layer chromatography tests, attention needs to be paid to selecting suitable chromatographic plates and other reagents to control the separation conditions of the samples.At the same time, it is also necessary to pay attention to the operation details in the chromatographic process, such as the accuracy of sampling and the uniformity of the solution.

Summary: Through the test methods of infrared spectroscopy, fluorescence colorimetry and thin layer chromatography, the content of anionic polyacrylamide can be accurately determined.In practical applications, it is necessary to choose the appropriate method according to the specific requirements of the experiment, and pay attention to the details of the operation process to ensure the accuracy and reliability of the test results.